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dna microarray analysis of yeast global gene expression  (RUCDR Infinite Biologics)

 
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    RUCDR Infinite Biologics dna microarray analysis of yeast global gene expression
    (a) Sod1 is required for the induction of oxidative response (OR) genes. WT or sod1 Δ cells were treated without or with 0.4 mM H 2 O 2 for 20 min and analyzed for <t>global</t> <t>gene</t> <t>expression</t> profile. 123 Sod1-dependent genes were identified and most of the known genes belong to five related functional categories. (b) Shown is the relative induction level of OR genes by H 2 O 2 in each category in WT and sod1 Δ cells. Data represents average fold change of induction in each category. (c) Shown is the heat map of genes in the oxidative stress response category. (d) Validation of representative genes ( GRE2 , Genes de Respuesta a Estres 2; TSA2 : Thiol-Specific Antioxidant 2; YML131; STF2 : STabilizing Factor 2) in the oxidative stress response category by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (e) Nuclear Sod1 is critical for the induction of OR genes. <t>Yeast</t> cells expressing different forms of Sod1 were treated with 0.4 mM H 2 O 2 for 20 min. Representative genes were validated by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (f) The induction of OR genes by ROS was attenuated in Sod1 S60,99A cells. Yeast cells expressing Sod1 or Sod1 S60,99A were treated with 0.4 mM H 2 O 2 for 20 min. Expression of GRE2 and RNR3 were determined by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (g) ROS treatment increases the association of Sod1 with promoter of oxidative responsive genes. WT (SZy1051) and sod1 Δ (SZy1050) cells were treated with 0.4 mM H 2 O 2 for 20 min. The binding of Sod1 to representative promoters were analyzed by chromatin immunoprecipitation (ChIP). (h) Quantification of the Fig. 5g experiment. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05, Student’s t -test.
    Dna Microarray Analysis Of Yeast Global Gene Expression, supplied by RUCDR Infinite Biologics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+microarray+analysis+of+yeast+global+gene+expression/dna+microarray+analysis+of+yeast+global+gene+expression/pmc04678626-194-4-13
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    1) Product Images from "Superoxide dismutase 1 acts as a nuclear transcription factor to regulate oxidative stress resistance"

    Article Title: Superoxide dismutase 1 acts as a nuclear transcription factor to regulate oxidative stress resistance

    Journal: Nature communications

    doi: 10.1038/ncomms4446

    (a) Sod1 is required for the induction of oxidative response (OR) genes. WT or sod1 Δ cells were treated without or with 0.4 mM H 2 O 2 for 20 min and analyzed for global gene expression profile. 123 Sod1-dependent genes were identified and most of the known genes belong to five related functional categories. (b) Shown is the relative induction level of OR genes by H 2 O 2 in each category in WT and sod1 Δ cells. Data represents average fold change of induction in each category. (c) Shown is the heat map of genes in the oxidative stress response category. (d) Validation of representative genes ( GRE2 , Genes de Respuesta a Estres 2; TSA2 : Thiol-Specific Antioxidant 2; YML131; STF2 : STabilizing Factor 2) in the oxidative stress response category by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (e) Nuclear Sod1 is critical for the induction of OR genes. Yeast cells expressing different forms of Sod1 were treated with 0.4 mM H 2 O 2 for 20 min. Representative genes were validated by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (f) The induction of OR genes by ROS was attenuated in Sod1 S60,99A cells. Yeast cells expressing Sod1 or Sod1 S60,99A were treated with 0.4 mM H 2 O 2 for 20 min. Expression of GRE2 and RNR3 were determined by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (g) ROS treatment increases the association of Sod1 with promoter of oxidative responsive genes. WT (SZy1051) and sod1 Δ (SZy1050) cells were treated with 0.4 mM H 2 O 2 for 20 min. The binding of Sod1 to representative promoters were analyzed by chromatin immunoprecipitation (ChIP). (h) Quantification of the Fig. 5g experiment. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05, Student’s t -test.
    Figure Legend Snippet: (a) Sod1 is required for the induction of oxidative response (OR) genes. WT or sod1 Δ cells were treated without or with 0.4 mM H 2 O 2 for 20 min and analyzed for global gene expression profile. 123 Sod1-dependent genes were identified and most of the known genes belong to five related functional categories. (b) Shown is the relative induction level of OR genes by H 2 O 2 in each category in WT and sod1 Δ cells. Data represents average fold change of induction in each category. (c) Shown is the heat map of genes in the oxidative stress response category. (d) Validation of representative genes ( GRE2 , Genes de Respuesta a Estres 2; TSA2 : Thiol-Specific Antioxidant 2; YML131; STF2 : STabilizing Factor 2) in the oxidative stress response category by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (e) Nuclear Sod1 is critical for the induction of OR genes. Yeast cells expressing different forms of Sod1 were treated with 0.4 mM H 2 O 2 for 20 min. Representative genes were validated by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (f) The induction of OR genes by ROS was attenuated in Sod1 S60,99A cells. Yeast cells expressing Sod1 or Sod1 S60,99A were treated with 0.4 mM H 2 O 2 for 20 min. Expression of GRE2 and RNR3 were determined by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (g) ROS treatment increases the association of Sod1 with promoter of oxidative responsive genes. WT (SZy1051) and sod1 Δ (SZy1050) cells were treated with 0.4 mM H 2 O 2 for 20 min. The binding of Sod1 to representative promoters were analyzed by chromatin immunoprecipitation (ChIP). (h) Quantification of the Fig. 5g experiment. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05, Student’s t -test.

    Techniques Used: Gene Expression, Functional Assay, Biomarker Discovery, Quantitative RT-PCR, Expressing, Binding Assay, Chromatin Immunoprecipitation

    Related Articles

    Microarray:

    Article Title: Superoxide dismutase 1 acts as a nuclear transcription factor to regulate oxidative stress resistance
    Article Snippet: RNA concentration was determined spectrophotometrically by Nanodrop 2000C. .. DNA microarray analysis of yeast global gene expression was carried out by Rutgers RUCDR Analytical and Informatics Services using the following procedure. .. The quality of purified RNA was assessed by Agilent Bioanalyzer 2100 using the Agilent RNA 6000 Nano Kit.

    Gene Expression:

    Article Title: Superoxide dismutase 1 acts as a nuclear transcription factor to regulate oxidative stress resistance
    Article Snippet: RNA concentration was determined spectrophotometrically by Nanodrop 2000C. .. DNA microarray analysis of yeast global gene expression was carried out by Rutgers RUCDR Analytical and Informatics Services using the following procedure. .. The quality of purified RNA was assessed by Agilent Bioanalyzer 2100 using the Agilent RNA 6000 Nano Kit.



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    dna microarray analysis of yeast global gene expression  (RUCDR Infinite Biologics)
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    RUCDR Infinite Biologics dna microarray analysis of yeast global gene expression
    (a) Sod1 is required for the induction of oxidative response (OR) genes. WT or sod1 Δ cells were treated without or with 0.4 mM H 2 O 2 for 20 min and analyzed for <t>global</t> <t>gene</t> <t>expression</t> profile. 123 Sod1-dependent genes were identified and most of the known genes belong to five related functional categories. (b) Shown is the relative induction level of OR genes by H 2 O 2 in each category in WT and sod1 Δ cells. Data represents average fold change of induction in each category. (c) Shown is the heat map of genes in the oxidative stress response category. (d) Validation of representative genes ( GRE2 , Genes de Respuesta a Estres 2; TSA2 : Thiol-Specific Antioxidant 2; YML131; STF2 : STabilizing Factor 2) in the oxidative stress response category by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (e) Nuclear Sod1 is critical for the induction of OR genes. <t>Yeast</t> cells expressing different forms of Sod1 were treated with 0.4 mM H 2 O 2 for 20 min. Representative genes were validated by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (f) The induction of OR genes by ROS was attenuated in Sod1 S60,99A cells. Yeast cells expressing Sod1 or Sod1 S60,99A were treated with 0.4 mM H 2 O 2 for 20 min. Expression of GRE2 and RNR3 were determined by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (g) ROS treatment increases the association of Sod1 with promoter of oxidative responsive genes. WT (SZy1051) and sod1 Δ (SZy1050) cells were treated with 0.4 mM H 2 O 2 for 20 min. The binding of Sod1 to representative promoters were analyzed by chromatin immunoprecipitation (ChIP). (h) Quantification of the Fig. 5g experiment. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05, Student’s t -test.
    Dna Microarray Analysis Of Yeast Global Gene Expression, supplied by RUCDR Infinite Biologics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+microarray+analysis+of+yeast+global+gene+expression/dna+microarray+analysis+of+yeast+global+gene+expression/pmc04678626-194-4-13
    Average 90 stars, based on 1 article reviews
    dna microarray analysis of yeast global gene expression - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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    (a) Sod1 is required for the induction of oxidative response (OR) genes. WT or sod1 Δ cells were treated without or with 0.4 mM H 2 O 2 for 20 min and analyzed for global gene expression profile. 123 Sod1-dependent genes were identified and most of the known genes belong to five related functional categories. (b) Shown is the relative induction level of OR genes by H 2 O 2 in each category in WT and sod1 Δ cells. Data represents average fold change of induction in each category. (c) Shown is the heat map of genes in the oxidative stress response category. (d) Validation of representative genes ( GRE2 , Genes de Respuesta a Estres 2; TSA2 : Thiol-Specific Antioxidant 2; YML131; STF2 : STabilizing Factor 2) in the oxidative stress response category by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (e) Nuclear Sod1 is critical for the induction of OR genes. Yeast cells expressing different forms of Sod1 were treated with 0.4 mM H 2 O 2 for 20 min. Representative genes were validated by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (f) The induction of OR genes by ROS was attenuated in Sod1 S60,99A cells. Yeast cells expressing Sod1 or Sod1 S60,99A were treated with 0.4 mM H 2 O 2 for 20 min. Expression of GRE2 and RNR3 were determined by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (g) ROS treatment increases the association of Sod1 with promoter of oxidative responsive genes. WT (SZy1051) and sod1 Δ (SZy1050) cells were treated with 0.4 mM H 2 O 2 for 20 min. The binding of Sod1 to representative promoters were analyzed by chromatin immunoprecipitation (ChIP). (h) Quantification of the Fig. 5g experiment. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05, Student’s t -test.

    Journal: Nature communications

    Article Title: Superoxide dismutase 1 acts as a nuclear transcription factor to regulate oxidative stress resistance

    doi: 10.1038/ncomms4446

    Figure Lengend Snippet: (a) Sod1 is required for the induction of oxidative response (OR) genes. WT or sod1 Δ cells were treated without or with 0.4 mM H 2 O 2 for 20 min and analyzed for global gene expression profile. 123 Sod1-dependent genes were identified and most of the known genes belong to five related functional categories. (b) Shown is the relative induction level of OR genes by H 2 O 2 in each category in WT and sod1 Δ cells. Data represents average fold change of induction in each category. (c) Shown is the heat map of genes in the oxidative stress response category. (d) Validation of representative genes ( GRE2 , Genes de Respuesta a Estres 2; TSA2 : Thiol-Specific Antioxidant 2; YML131; STF2 : STabilizing Factor 2) in the oxidative stress response category by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (e) Nuclear Sod1 is critical for the induction of OR genes. Yeast cells expressing different forms of Sod1 were treated with 0.4 mM H 2 O 2 for 20 min. Representative genes were validated by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (f) The induction of OR genes by ROS was attenuated in Sod1 S60,99A cells. Yeast cells expressing Sod1 or Sod1 S60,99A were treated with 0.4 mM H 2 O 2 for 20 min. Expression of GRE2 and RNR3 were determined by RT-qPCR. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05. (g) ROS treatment increases the association of Sod1 with promoter of oxidative responsive genes. WT (SZy1051) and sod1 Δ (SZy1050) cells were treated with 0.4 mM H 2 O 2 for 20 min. The binding of Sod1 to representative promoters were analyzed by chromatin immunoprecipitation (ChIP). (h) Quantification of the Fig. 5g experiment. Error bars indicate ± SD from triplicates of two independent experiments. * p < 0.05, Student’s t -test.

    Article Snippet: DNA microarray analysis of yeast global gene expression was carried out by Rutgers RUCDR Analytical and Informatics Services using the following procedure.

    Techniques: Gene Expression, Functional Assay, Biomarker Discovery, Quantitative RT-PCR, Expressing, Binding Assay, Chromatin Immunoprecipitation